Composite

Part:BBa_K3096044:Design

Designed by: Patrick Mueller, Famke Baeuerle, Lina Widerspick, Jakob Keck   Group: iGEM19_Tuebingen   (2019-10-15)


Glucose dependent expression system of Exendin-4 with a N-terminal secretion tag and a CPP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1297
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Removal of unwanted cut sites, in frame Ligation and proper distance between the Segments.


Source

Combination of previously designed parts.

References